Coagulation Panel

CPT Code(s):

  • 85610
  • 85730

Test Component(s):

  • Prothrombin time with INR
  • aPTT

Preferred Specimen:

One full unopened 3.2% sodium citrate (light blue-top) tube

Minimum Volume:

Ratio of blood to citrate is critical. Submit difficult draws in pediatric tube. 0.5 mL frozen plasma

Alternate Specimen:

1 mL frozen plasma:If the specimen will be delayed longer than 6 hours, centrifuge specimen for 15 minutes at 2500-3500 rpm. Using a plastic pipette, remove plasma, taking care to avoid the WBC/ platelet (buffy) layer and place into a plastic screw-cap vial and freeze at -20¡C. Ship on dry ice.

Special Instructions:

A completely filled tube is necessary because the correct ratio of blood to citrate is critical (9:1). Mix by gentle inversion 3-4 times. Do not uncap.

Transport Container:

Whole blood: 3.2% sodium citrate (light blue-top) tube Plasma: Plastic screw-cap vial (frozen)

Transport Temperature Name:

Room temperature: Light blue-top tube (whole blood) Frozen: Plasma

Specimen Stability Components:

  • Room Temperature: Unopened light blue-top tube Room temperature: 6 hours Refrigerated: 24 hours Frozen: Unacceptable Separated platelet-poor plasma Room temperature: 4 hours Refrigerated: 24 hours Frozen -20¡C: 2 days

Testing Schedule:

Daily

Methodology:

Mechanical Clot Detection

Reject Criteria:

Gross hemolysis, gross lipemia, improper blood to citrate ratio, high hematocrit, improper blood collection

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Genesis