- Prothrombin time with INR
One full unopened 3.2% sodium citrate (light blue-top) tube
Ratio of blood to citrate is critical. Submit difficult draws in pediatric tube. 0.5 mL frozen plasma
1 mL frozen plasma:If the specimen will be delayed longer than 6 hours, centrifuge specimen for 15 minutes at 2500-3500 rpm. Using a plastic pipette, remove plasma, taking care to avoid the WBC/ platelet (buffy) layer and place into a plastic screw-cap vial and freeze at -20¡C. Ship on dry ice.
A completely filled tube is necessary because the correct ratio of blood to citrate is critical (9:1). Mix by gentle inversion 3-4 times. Do not uncap.
Whole blood: 3.2% sodium citrate (light blue-top) tube Plasma: Plastic screw-cap vial (frozen)
Transport Temperature Name:
Room temperature: Light blue-top tube (whole blood) Frozen: Plasma
Specimen Stability Components:
- Room Temperature: Unopened light blue-top tube Room temperature: 6 hours Refrigerated: 24 hours Frozen: Unacceptable Separated platelet-poor plasma Room temperature: 4 hours Refrigerated: 24 hours Frozen -20¡C: 2 days
Mechanical Clot Detection
Gross hemolysis, gross lipemia, improper blood to citrate ratio, high hematocrit, improper blood collection