CBC with Manual Differential

CPT Code(s):

  • 85027
  • 85077
  • 85060

Preferred Specimen:

Whole blood collected in an EDTA (lavender-top) tube

Minimum Volume:

2.5 mL whole blood EDTA (lavender-top) tube

Special Instructions:

Maintain specimen at room temperature. Do not refrigerate. If multiple draw, collect EDTA (lavender-top) tube last. Traumatic draw can introduce thromboplastin and trap WBC and platelets. Refrigeration can precipitate fibrin and trap WBC and platelets.

Transport Container:

EDTA (lavender-top) tube

Transport Temperature Name:

Room temperature

Specimen Stability Components:

  • Room Temperature: 24 hours
  • Refrigerated: 24 hours (may cause platelet clumping)

Testing Schedule:


Reject Criteria:

Hemolysis ¥ Clotted ¥ Received Frozen

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